2. Tool installation¶
2.1. Install the conda package manager¶
We will use the package/tool managing system conda to install some programs that we will use during the course. It is not installed by default, thus we need to install it first to be able to use it.
# download latest conda installer curl -O https://repo.continuum.io/miniconda/Miniconda3-latest-Linux-x86_64.sh # run the installer bash Miniconda3-latest-Linux-x86_64.sh # delete the installer after successful run rm Miniconda3-latest-Linux-x86_64.sh
Should the conda installer download fail. Please find links to alternative locations on the Downloads page.
echo 'export PATH="/home/manager/miniconda3/bin:$PATH"' >> ~/.bashrc echo 'export PATH="/home/manager/miniconda3/bin:$PATH"' >> ~/.zshrc
The above assumes that your username is “manager”, which is the default on a Biolinux install.
Replace “manager” with your actual username.
Find out with
So what is actually happening here? We are appending a line to a file (either
If we are starting a new command-line shell, either file gets executed first (depending on which shell you are using, either bash or zsh shells).
What this line does, is to put permanently the directory
~/miniconda3/bin first on your
PATH variable contains directories in which our computer looks for installed programs, one directory after the other until the program you requested is found (or not, then it will complain).
Through the addition of the above line we make sure that the program
conda can be found anytime we open a new shell.
Close shell/terminal, re-open new shell/terminal. Now, we should be able to use the conda command:
conda update conda
2.1.2. Installing conda channels to make tools available¶
Different tools are packaged in what conda calls channels. We need to add some channels to make the bioinformatics and genomics tools available for installation:
# Install some conda channels # A channel is where conda looks for packages conda config --add channels defaults conda config --add channels conda-forge conda config --add channels bioconda
2.2. Create environments¶
We create a conda environment for some tools This is useful to work reproducible as we can easily re-create the tool-set with the same version numbers later on.
conda create -n ngs python=3 # activate the environment conda activate ngs
So what is happening when you type
conda activate ngs in a shell.
PATH variable (mentioned above) gets temporarily manipulated and set to:
$ conda activate ngs # Lets look at the content of the PATH variable (ngs) $ echo $PATH /home/manager/miniconda3/envs/ngs/bin:/home manager/miniconda3/bin:/usr/local/bin: ...
Now it will look first in your environment’s bin directory but afterwards in the general conda bin (/home/manager/miniconda3/bin).
So basically everything you install generally with conda (without being in an environment) is also available to you but gets overshadowed if a similar program is in
/home/manager/miniconda3/envs/ngs/bin and you are in the
2.3. Install software¶
To install software into the activated environment, one uses the command
# install more tools into the environment conda install package
To tell if you are in the correct conda environment, look at the command-prompt.
Do you see the name of the environment in round brackets at the very beginning of the prompt, e.g. (ngs)?
If not, activate the
ngs environment with
conda activate ngs before installing the tools.
2.4. General conda commands¶
# to search for packages conda search [package] # To update all packages conda update --all --yes # List all packages installed conda list [-n env] # conda list environments conda env list # create new env conda create -n [name] package [package] ... # activate env conda activate [name] # deavtivate env conda deactivate